DPCfam Metaclusters

🧬 Domain Classification in Sequence Space
DPCfam Standard DPCfam DPCfamB

The DPCfam pipeline applied the Density Peak Clustering (DPC) algorithm to roughly 23 million UniRef50 sequences (UniRef50 v. 2017_07) and automatically grouped homologous domain segments into 81,384 protein families called metaclusters, entirely without manual curation. Each metacluster was then used to build a multiple sequence alignment and a profile-HMM for downstream analysis.

The table below lists all 81,384 DPCfam metaclusters by default. Use the toggle buttons above to switch to the Standard subset (size ≥ 50 seeds, 46,828 metaclusters) or the DPCfamB subset (25 to 49 seeds, 34,556 metaclusters). Intrinsic metacluster properties appear on the left; Pfam annotations on the right. Click any MCID to open its detail page.

MCID Size UniRef50 Avg. Len. Std avg len % LC % CC % DIS Avg. TM Pfam DA % DA % Avg. Ov. Overlap Label
MC49817 133 76.0 15.16 9.05 0.0 35.34 0.01 PF06404 98.08 72.91 Extended
MC49820 147 176.17 23.49 2.5 0.0 20.06 0.0 PF14103 80.34 90.9 Equivalent
MC49843 51 792.47 40.14 4.36 0.17 21.96 0.33 PF10487-PF10487 52.0 59.77 Reduced
MC49844 169 53.82 3.4 0.78 0.0 12.15 0.0 PF14255 100.0 86.27 Equivalent
MC49849 67 141.48 10.0 5.34 0.0 30.23 0.0 PF09206 100.0 49.13 Reduced
MC49855 62 185.73 20.0 8.94 1.08 23.71 0.02 UNKNOWN 0.0 0.0 NONE
MC49858 55 75.29 10.88 9.12 0.0 59.62 0.0 UNKNOWN 0.0 0.0 NONE
MC49859 50 212.94 34.54 18.41 0.0 70.34 0.0 UNKNOWN 0.0 0.0 NONE
MC49861 131 81.3 8.49 0.6 0.0 26.89 0.0 PF14033 99.22 10.8 Shifted
MC49866 56 88.93 10.61 1.02 0.0 29.2 0.0 PF14010 100.0 29.53 Reduced
Legend
  • A. Metacluster Intrinsic Properties:
  • MCID: Unique identifier per metacluster.
  • 1. Size UniRef50 : Number of UniRef50 seed sequences in the metacluster.
  • 2. Avg. Len.: Mean domain length of the seeds (in amino acids).
  • 3. Std. Avg. Len.: Standard deviation of seed domain lengths.
  • 4. % LC Percent of residues located in low-complexity regions.
  • 5. % CC: Percent of residues located in coiled-coil regions.
  • 6. % DIS: Percent of residues predicted as intrinsically disordered.
  • 7. Avg.TM: Average number of transmembrane regions per domain.
  • B. Pfam Annotations:
  • 1. Pfam DA: Pfam architecture which best overlaps with MC. We refer to it as the dominant architecture (DA). If there is no overlap, the MC is tagged as UNKNOWN (representing 'dark matter').
  • 2. % DA: Percent of sequences in the MC matching the DA.
  • 3. % Avg. Ov.: Average % boundary overlap between the MC and Pfam, computed only for seeds that match the DA.
  • 4. Overlap Label: Label describing qualitatively the overlap of the MC with its DA, which can be : equivalent, reduced, extended or shifted. NONE, otherwise.
    Color-coded based on boundary agreement:
    • Yellow (Equivalent): the MC and its dominant architecture share very similar boundaries.
    • Blue (Reduced): the MC covers only part of the dominant architecture.
    • Pink (Extended): the MC extends beyond the dominant architecture boundaries.
    • Green (Shifted): the MC and the dominant architecture share a boundary offset, with poor positional agreement.