DPCfam Metaclusters

🧬 Domain Classification in Sequence Space
DPCfam Standard DPCfam DPCfamB

The DPCfam pipeline applied the Density Peak Clustering (DPC) algorithm to roughly 23 million UniRef50 sequences (UniRef50 v. 2017_07) and automatically grouped homologous domain segments into 81,384 protein families called metaclusters, entirely without manual curation. Each metacluster was then used to build a multiple sequence alignment and a profile-HMM for downstream analysis.

The table below lists all 81,384 DPCfam metaclusters by default. Use the toggle buttons above to switch to the Standard subset (size ≥ 50 seeds, 46,828 metaclusters) or the DPCfamB subset (25 to 49 seeds, 34,556 metaclusters). Intrinsic metacluster properties appear on the left; Pfam annotations on the right. Click any MCID to open its detail page.

MCID Size UniRef50 Avg. Len. Std avg len % LC % CC % DIS Avg. TM Pfam DA % DA % Avg. Ov. Overlap Label
MC30113 93 91.1 8.17 1.68 0.0 10.31 0.17 PF00211 94.51 47.13 Reduced
MC30115 61 67.33 5.75 1.32 0.0 49.03 0.0 PF00130 3.33 72.73 Extended
MC30130 126 189.73 31.67 2.18 0.0 20.47 0.06 UNKNOWN 0.0 0.0 NONE
MC30132 65 140.01 14.6 1.72 0.0 23.96 0.02 PF00722 87.3 19.26 Shifted
MC30133 150 52.67 8.74 0.95 0.0 40.77 0.0 PF13442 63.41 37.66 Shifted
MC30140 340 446.74 61.97 7.39 0.0 21.92 0.1 PF00881 44.91 29.95 Extended
MC30144 103 616.18 136.07 8.45 0.1 42.83 0.05 PF16496-PF04433 32.53 90.76 Equivalent
MC30158 76 237.51 35.99 1.34 3.03 14.9 0.0 UNKNOWN 0.0 0.0 NONE
MC30164 63 76.37 7.13 3.77 0.0 27.49 0.0 PF01022 14.89 42.02 Extended
MC30168 53 64.25 5.66 0.82 0.0 16.04 0.0 PF10151 65.91 20.15 Reduced
Legend
  • A. Metacluster Intrinsic Properties:
  • MCID: Unique identifier per metacluster.
  • 1. Size UniRef50 : Number of UniRef50 seed sequences in the metacluster.
  • 2. Avg. Len.: Mean domain length of the seeds (in amino acids).
  • 3. Std. Avg. Len.: Standard deviation of seed domain lengths.
  • 4. % LC Percent of residues located in low-complexity regions.
  • 5. % CC: Percent of residues located in coiled-coil regions.
  • 6. % DIS: Percent of residues predicted as intrinsically disordered.
  • 7. Avg.TM: Average number of transmembrane regions per domain.
  • B. Pfam Annotations:
  • 1. Pfam DA: Pfam architecture which best overlaps with MC. We refer to it as the dominant architecture (DA). If there is no overlap, the MC is tagged as UNKNOWN (representing 'dark matter').
  • 2. % DA: Percent of sequences in the MC matching the DA.
  • 3. % Avg. Ov.: Average % boundary overlap between the MC and Pfam, computed only for seeds that match the DA.
  • 4. Overlap Label: Label describing qualitatively the overlap of the MC with its DA, which can be : equivalent, reduced, extended or shifted. NONE, otherwise.
    Color-coded based on boundary agreement:
    • Yellow (Equivalent): the MC and its dominant architecture share very similar boundaries.
    • Blue (Reduced): the MC covers only part of the dominant architecture.
    • Pink (Extended): the MC extends beyond the dominant architecture boundaries.
    • Green (Shifted): the MC and the dominant architecture share a boundary offset, with poor positional agreement.