DPCfam Metaclusters

🧬 Domain Classification in Sequence Space
DPCfam Standard DPCfam DPCfamB

The DPCfam pipeline applied the Density Peak Clustering (DPC) algorithm to roughly 23 million UniRef50 sequences (UniRef50 v. 2017_07) and automatically grouped homologous domain segments into 81,384 protein families called metaclusters, entirely without manual curation. Each metacluster was then used to build a multiple sequence alignment and a profile-HMM for downstream analysis.

The table below lists all 81,384 DPCfam metaclusters by default. Use the toggle buttons above to switch to the Standard subset (size ≥ 50 seeds, 46,828 metaclusters) or the DPCfamB subset (25 to 49 seeds, 34,556 metaclusters). Intrinsic metacluster properties appear on the left; Pfam annotations on the right. Click any MCID to open its detail page.

MCID Size UniRef50 Avg. Len. Std avg len % LC % CC % DIS Avg. TM Pfam DA % DA % Avg. Ov. Overlap Label
MC225428 43 393.09 45.46 0.04 0.0 0.76 0.0 PF00646 13.95 8.44 Extended
MC225462 31 151.26 13.69 0.23 0.0 0.73 3.65 UNKNOWN 0.0 0.0 NONE
MC225476 27 57.85 4.66 0.02 1.17 1.98 0.0 PF05119 68.18 54.33 Reduced
MC225481 46 160.04 15.15 0.01 0.0 0.35 0.0 UNKNOWN 0.0 0.0 NONE
MC225498 49 64.67 8.66 0.56 0.0 33.66 0.0 UNKNOWN 0.0 0.0 NONE
MC225502 31 262.48 46.83 0.06 0.0 7.15 0.12 PF13560 15.79 8.3 Shifted
MC225522 28 245.36 18.47 0.07 0.0 0.29 4.04 UNKNOWN 0.0 0.0 NONE
MC225535 45 105.84 10.36 0.05 0.0 30.05 0.0 PF01530-PF08474 78.57 44.55 Reduced
MC225554 25 140.36 12.08 0.01 0.0 0.86 0.0 PF09556 100.0 44.75 Reduced
MC225559 39 276.95 21.76 0.02 0.0 0.0 0.1 PF08270 93.1 53.24 Extended
Legend
  • A. Metacluster Intrinsic Properties:
  • MCID: Unique identifier per metacluster.
  • 1. Size UniRef50 : Number of UniRef50 seed sequences in the metacluster.
  • 2. Avg. Len.: Mean domain length of the seeds (in amino acids).
  • 3. Std. Avg. Len.: Standard deviation of seed domain lengths.
  • 4. % LC Percent of residues located in low-complexity regions.
  • 5. % CC: Percent of residues located in coiled-coil regions.
  • 6. % DIS: Percent of residues predicted as intrinsically disordered.
  • 7. Avg.TM: Average number of transmembrane regions per domain.
  • B. Pfam Annotations:
  • 1. Pfam DA: Pfam architecture which best overlaps with MC. We refer to it as the dominant architecture (DA). If there is no overlap, the MC is tagged as UNKNOWN (representing 'dark matter').
  • 2. % DA: Percent of sequences in the MC matching the DA.
  • 3. % Avg. Ov.: Average % boundary overlap between the MC and Pfam, computed only for seeds that match the DA.
  • 4. Overlap Label: Label describing qualitatively the overlap of the MC with its DA, which can be : equivalent, reduced, extended or shifted. NONE, otherwise.
    Color-coded based on boundary agreement:
    • Yellow (Equivalent): the MC and its dominant architecture share very similar boundaries.
    • Blue (Reduced): the MC covers only part of the dominant architecture.
    • Pink (Extended): the MC extends beyond the dominant architecture boundaries.
    • Green (Shifted): the MC and the dominant architecture share a boundary offset, with poor positional agreement.